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KMID : 0525619980030020165
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1998 Volume.3 No. 2 p.165 ~ p.175
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Uitilization of Immobilized Enzyme for Producing Effectively Chitosan Oligosaccharides
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전유진/Jeon YJ
김세권/Kim SK
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Abstract
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Enzymatic hydrolysis using an immobilized enzyme was carried out to produce chitosan oligosaccharides(COSs)from chitosan effectively. Chitosanase immobilized on eight different carriers by Physical adsorption. The enzyme immobilized on chitin had higher activity than those immobilized on the other carriers in spite of its lower adsorption. The activity of chitin-immobilized enzyme was more than 90% of the original activity. Optimal temperature of the immobilized enzyme increased by about 15℃ and its thermostability was excellent in relatively wide range of temperature. But its effects on pH did not improve compared to the free enzyme. The immobilized enzyme produced 153 mg/g chitosan of the reducing sugar for 3hrs of hydrolytic incubation time. The total content of higher oligorners, tetramer to hexamer, among amount of total COSs obtained for 2hrs was more than 90%. In kinetic parameters for both enzymes, immobilized enzyme showed lower affinity for substrate and reaction rate than free enzyme, however, no reduction of the rate for high substrate concentrations. Consequently, chitin-immobilized enzyme could effectively hydrolyse chitosan and produce the higher COSs without activity decrease in comparison with the free enzyme.
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